5USW
The crystal structure of 7,8-dihydropteroate synthase from Vibrio fischeri ES114
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-08 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.953, 65.881, 94.942 |
| Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
| Resolution | 36.130 - 1.643 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jq9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.055 | 0.831 |
| Rpim | 0.037 | 0.592 |
| Number of reflections | 130804 | 6479 |
| <I/σ(I)> | 13.8 | 1.5 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M potassium sodium tartrate, 20% w/v PEG3350 |
