5UQY

Crystal structure of Marburg virus GP in complex with the human survivor antibody MR78

Replaces:  3X2D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-02
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	P 63
Unit cell lengths	204.653, 204.653, 192.963
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.739 - 3.600
R-factor	0.2233
Rwork	0.221
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3csy
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	130.530	3.612
High resolution limit [Å]	3.600	3.600
Number of reflections	52806	534
<I/σ(I)>	12.9	2.1
Completeness [%]	99.4	100
Redundancy	5.1	5.1
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M NaCl, 0.05 M MES pH6.5, 13 % PEG4000, 0.5 % ethyl acetate