5ULH

Structure of RNF165 in complex with a UbcH5b~Ub conjugate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	58.278, 60.081, 112.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.170 - 1.950
R-factor	0.1903
Rwork	0.189
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.790
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.170
High resolution limit [Å]	1.950
Rmerge	0.097
Number of reflections	29425
<I/σ(I)>	15
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	200 mM potassium thiocyanate, 20% PEG 3350