5UD7

Crystal Structure of Wild-Type Ig-like Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-24
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	160.664, 160.664, 86.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.493 - 2.200
R-factor	0.242745918946
Rwork	0.241
R-free	0.27748
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ud8
RMSD bond length	0.009
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.493	47.490	2.260
High resolution limit [Å]	2.200	9.590	2.200
Rmerge	0.266	0.059	3.535
Number of reflections	57945
<I/σ(I)>	14.1
Completeness [%]	100.0	99.3	100
Redundancy	25.6	19.7	27.6
CC(1/2)	0.999	0.999	0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.9	293	0.2 M sodium iodide, 1.6 M ammonium sulfate, 0.1 M Tris 7.9