5U8C

CRYSTAL STRUCTURE OF GLUN1/GLUN2A LIGAND-BINDING DOMAIN IN COMPLEX WITH GLYCINE AND NVP-AAM077

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	59.550, 84.227, 121.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.196 - 1.598
R-factor	0.1893
Rwork	0.189
R-free	0.20410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nf8
RMSD bond length	0.008
RMSD bond angle	0.930
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.598
Rmerge	0.077
Number of reflections	72757
<I/σ(I)>	17.84
Completeness [%]	89.5
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	18% Polyethylene glycol monoethylether 2000 (PEG2000 MME), 100mM HEPES-NaOH pH 7.0, and 75mM NaCl