5U84
Acid ceramidase (ASAH1, aCDase) from common minke whale, Cys143Ala, uncleaved
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.77122 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.815, 109.267, 79.541 |
| Unit cell angles | 90.00, 102.97, 90.00 |
Refinement procedure
| Resolution | 44.656 - 2.340 |
| R-factor | 0.2014 |
| Rwork | 0.200 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.727 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.656 |
| High resolution limit [Å] | 2.340 |
| Number of reflections | 67018 |
| <I/σ(I)> | 14.4 |
| Completeness [%] | 99.0 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | citric acid sodium chloride lithium 5-amino-2,4,6-triiodoisophthalate (I3C) |
