5U18

Crystal structure of a methyltransferase involved in the biosynthesis of gentamicin in complex with the Geneticin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X3A
Synchrotron site	NSLS
Beamline	X3A
Temperature [K]	293
Detector technology	PIXEL
Collection date	2013-11-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	65.113, 68.892, 69.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.854 - 2.195
R-factor	0.1731
Rwork	0.171
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.854
High resolution limit [Å]	2.195
Number of reflections	16371
<I/σ(I)>	13.64
Completeness [%]	99.2
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Magnesium chloride, tris pH 8, peg 3350 and peg 400