5TZT

Crystal structure of human CD47 ECD bound to Fab of C47B161

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	74.569, 60.974, 124.133
Unit cell angles	90.00, 90.05, 90.00

Refinement procedure

Resolution	41.378 - 2.890
R-factor	0.2609
Rwork	0.257
R-free	0.32790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jjs
RMSD bond length	0.005
RMSD bond angle	1.016
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.378	2.950
High resolution limit [Å]	2.890	2.890
Rmerge	0.119	0.465
Number of reflections	24865
<I/σ(I)>	6.9
Completeness [%]	98.6	90.9
Redundancy	3.1	2.9
CC(1/2)		0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	34% PEG 8000, 0.1M HEPES pH 7.5