5TUF

Crystal structure of tetracycline destructase Tet(50) in complex with anhydrotetracycline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-10-21
Detector	RDI CMOS_8M
Wavelength(s)	1.000031
Spacegroup name	P 21 21 21
Unit cell lengths	50.990, 107.370, 152.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.791 - 2.250
R-factor	0.2084
Rwork	0.207
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.742
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1616)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.250	2.250
Number of reflections	40157
<I/σ(I)>	13.57
Completeness [%]	98.6
Redundancy	4.93
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES, pH 6.0-6.5, 1.6-2.0 M ammonium sulfate, 2-10% 1,4-dioxane