5TMA
Zymomonas mobilis pyruvate decarboxylase mutant PDC-2.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-31 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54188 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.440, 124.440, 173.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.180 - 1.670 |
| R-factor | 0.17201 |
| Rwork | 0.170 |
| R-free | 0.20269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.942 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.180 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.787 | |
| Number of reflections | 157603 | |
| <I/σ(I)> | 16.57 | 16.57 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 10.41 | 4.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES monohydrate pH 6.0, 2.4 M ammonium sulfate |
