5TL7
Crystal structure of SARS-CoV papain-like protease in complex with C-terminal domain mouse ISG15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-26 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.981, 98.191, 106.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.230 - 2.440 |
| R-factor | 0.19615 |
| Rwork | 0.192 |
| R-free | 0.26744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e9s 5jze |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.230 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.099 | 0.742 |
| Number of reflections | 29922 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.1 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 65% (v/v) MPD, 0.1 M Tris [pH 8.0], supplemented with 30% (w/v) Trimethylamine N-oxide dihydrate additive in a 1:5 dilution |
