5TL7

Crystal structure of SARS-CoV papain-like protease in complex with C-terminal domain mouse ISG15

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Ubiquitin-like protein ISG15	polymer	79	9280.7	2	UniProt (Q64339) Pfam (PF00240) In PDB	Mus musculus (Mouse)	Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein,IP17,Ubiquitin cross-reactive protein
2	B, D	Replicase polyprotein 1ab	polymer	319	35809.6	2	UniProt (P0C6X7) Pfam (PF08715) In PDB	SARS coronavirus (SARS-CoV)	pp1ab,ORF1ab polyprotein
3	B, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4		water	water		18.0	122	Chemie (HOH)

Sequence modifications

A, C: 78 - 155 (UniProt: Q64339)

PDB	External Database	Details
Met 77	-	initiating methionine
Aye 155	Gly 155	engineered mutation

B, D: 2 - 316 (UniProt: P0C6X7)

PDB	External Database	Details
Met -2	-	initiating methionine
Ala -1	-	expression tag
Ser 0	-	expression tag
Met 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	90180.6
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	90311.5

*Water molecules are not included.