5TJ2
Gasdermin-B C-terminal domain containing the polymorphism residues Gly299:Ser306 fused to maltose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-11-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 144.039, 152.750, 255.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.500 - 2.800 |
| R-factor | 0.2153 |
| Rwork | 0.213 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tib |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.500 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.155 |
| Number of reflections | 65695 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 99.6 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 295 | 2.1 M sodium malonate, 10 mM EDTA |
