5TD5

Crystal Structure of Human APOBEC3B variant complexed with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 64 2 2
Unit cell lengths	96.411, 96.411, 84.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.494 - 1.718
R-factor	0.1816
Rwork	0.180
R-free	0.20890
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.750
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.494	1.780
High resolution limit [Å]	1.718	1.718
Rmerge	0.054	1.275
Number of reflections	50564
<I/σ(I)>	35.3	2.1
Completeness [%]	99.7	97.2
Redundancy	12.7	11.8
CC(1/2)	0.999	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	NaI, PEG3350