5TCG
Crystal structure of tryptophan synthase from M. tuberculosis - aminoacrylate-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.183, 159.433, 165.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.1724 |
| Rwork | 0.172 |
| R-free | 0.20614 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.178 | 0.820 |
| Number of reflections | 139260 | |
| <I/σ(I)> | 11.78 | 1.81 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 8.9 | 6 |
| CC(1/2) | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 8% tacsimate pH 8.0, 20% PEG3350, 3% trimethylamine N-oxide dihydrate, soaked in 80 mM CsCl, 30 mM L-Ser and 17% ethylene glycol |
