5T9J
Crystal Structure of human GEN1 in complex with Holliday junction DNA in the upper interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999950,0.978940,1.254730 |
| Spacegroup name | P 32 |
| Unit cell lengths | 86.940, 86.940, 200.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.290 - 3.000 |
| R-factor | 0.20088296201 |
| Rwork | 0.199 |
| R-free | 0.24062 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.623 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.290 | 3.140 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.907 | |
| Number of reflections | 34063 | |
| <I/σ(I)> | 18.4 | 1.9 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.27 | 6.42 |
| CC(1/2) | 0.998 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100 mM MES-NaOH, 200 mM NaCl, ratio sample:crystallizing agent 2:1 |
