5T89

Crystal structure of VEGF-A in complex with VEGFR-1 domains D1-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	166.710, 123.090, 167.370
Unit cell angles	90.00, 109.56, 90.00

Refinement procedure

Resolution	19.988 - 4.000
R-factor	0.286
Rwork	0.285
R-free	0.31530
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.710
Data reduction software	XDS (Version January 10, 2014)
Data scaling software	XSCALE (Version January 10, 2014)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (dev_2420)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		4.100
High resolution limit [Å]	4.000	17.890	4.000
Rmerge	0.180	0.026	2.002
Number of reflections	26988
<I/σ(I)>	9.53	52.15	1.18
Completeness [%]	99.5	94	99.3
Redundancy	6.9
CC(1/2)	0.998	0.999	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	10-14% PEG3000 200 mM calcium chloride 100 mM MES pH 6.5