5T87
Crystal structure of CDI complex from Cupriavidus taiwanensis LMG 19424
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791519 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.097, 107.097, 315.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.21613 |
| Rwork | 0.215 |
| R-free | 0.24697 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.143 | 0.693 |
| Number of reflections | 42464 | |
| <I/σ(I)> | 12.84 | 1.45 |
| Completeness [%] | 99.7 | 96.3 |
| Redundancy | 6.2 | 4.9 |
| CC(1/2) | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.1 M Mg acetate, 0.1 M Na acetate, 8% PEG 8000 |
