5T4I

A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.89
Spacegroup name	P 21 21 21
Unit cell lengths	73.508, 79.723, 138.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.031 - 2.389
R-factor	0.1928
Rwork	0.191
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t40
RMSD bond length	0.003
RMSD bond angle	0.526
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.389	6.510	2.389
Rmerge	0.114	0.057	0.638
Rmeas	0.126
Rpim	0.052
Total number of observations	352305
Number of reflections	32857
<I/σ(I)>	8.2
Completeness [%]	99.5	99.6	97.4
Redundancy	5.7	6.2	4.4
CC(1/2)		0.997	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	22% PEG 2000 MM 0.2M (NH4)2SO4, 0.1M Na Acetate pH 4.6 100mM MnCl2