5T46
Crystal structure of the human eIF4E-eIF4G complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.250, 70.347, 79.913 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 44.647 - 1.530 |
| R-factor | 0.1401 |
| Rwork | 0.139 |
| R-free | 0.16190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tpw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.650 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 1.013 | |
| Number of reflections | 74453 | |
| <I/σ(I)> | 11.6 | 1.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.998 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 293 | 0.1 M Hepes pH 7.25, 25% w/v PEG 6000 |
