5T46
Crystal structure of the human eIF4E-eIF4G complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.250, 70.347, 79.913 |
Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
Resolution | 44.647 - 1.530 |
R-factor | 0.1401 |
Rwork | 0.139 |
R-free | 0.16190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tpw |
RMSD bond length | 0.008 |
RMSD bond angle | 0.918 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2152)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.650 | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 1.013 | |
Number of reflections | 74453 | |
<I/σ(I)> | 11.6 | 1.2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.998 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 293 | 0.1 M Hepes pH 7.25, 25% w/v PEG 6000 |