5T2N

Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP007280 gene; harbors 38 point mutations relative to wild-type I-OnuI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	108
Detector technology	PIXEL
Collection date	2016-05-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.084
Spacegroup name	P 21 21 21
Unit cell lengths	40.834, 67.421, 166.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.908 - 2.079
R-factor	0.2226
Rwork	0.220
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qqy
RMSD bond length	0.003
RMSD bond angle	0.697
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.150
High resolution limit [Å]	2.080	4.480	2.080
Rmerge	0.094	0.072	0.793
Rmeas	0.098	0.076	0.839
Rpim	0.028	0.022	0.266
Total number of observations	339574
Number of reflections	28554
<I/σ(I)>	10.1
Completeness [%]	99.6	99.4	99
Redundancy	11.9	11.8	8.9
CC(1/2)		0.995	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.05M ammonium sulfate, 0.05M BIS-TRIS pH 6.5, 35% pentaerythritol ethoxylate (15/4 EO/OH)