5T00
Human CTCF ZnF3-7 and methylated DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.990, 44.910, 86.800 |
| Unit cell angles | 98.33, 92.40, 94.80 |
Refinement procedure
| Resolution | 41.956 - 2.190 |
| R-factor | 0.2196 |
| Rwork | 0.218 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kkq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.684 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2257: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.956 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.683 | |
| Number of reflections | 31307 | |
| <I/σ(I)> | 6.56 | 1.6 |
| Completeness [%] | 98.3 | 84.5 |
| Redundancy | 7.4 | 6.4 |
| CC(1/2) | 0.994 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 25% (w/v) PEG 3350, 0.2 M NaCl, 0.1 M HEPES-NaOH, pH 7.5 |
