5OY7
Structure of the 4_601_157 tetranucleosome (P1 form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 66.624, 161.465, 227.915 |
Unit cell angles | 78.94, 83.86, 83.74 |
Refinement procedure
Resolution | 111.407 - 5.774 |
R-factor | 0.2199 |
Rwork | 0.219 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lz0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.462 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 141.500 | |
High resolution limit [Å] | 5.770 | 5.770 |
Rmerge | 0.110 | |
Rmeas | 0.076 | 0.506 |
Number of reflections | 24331 | 4652 |
<I/σ(I)> | 4.9 | 1.63 |
Completeness [%] | 93.8 | 82 |
Redundancy | 1.9 | |
CC(1/2) | 0.996 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 30-60 mM KCl, 90-110 mM MgCl2 and 5 mM Na-cacodyalte, pH 6.0 |