5OVW

Nanobody-bound BtuF, the vitamin B12 binding protein in Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99984
Spacegroup name	P 21 21 21
Unit cell lengths	83.800, 141.000, 216.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.996 - 2.653
R-factor	0.2251
Rwork	0.223
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.558
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.996
High resolution limit [Å]	2.653
Number of reflections	63910
<I/σ(I)>	1.36
Completeness [%]	85.3
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	PEG4000, Tris-HCl, Magnesium chloride