5OTT

Extracellular domain of GLP-1 receptor in complex with exendin-4 variant Gly2Hcs/Thr5Hcs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-24
Detector	DECTRIS PILATUS3 R 1M
Wavelength(s)	1.54187
Spacegroup name	I 2 2 2
Unit cell lengths	51.690, 62.920, 118.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.382 - 1.920
R-factor	0.1824
Rwork	0.180
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zgm
RMSD bond length	0.015
RMSD bond angle	1.253
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2689: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.382	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.103	1.145
Rmeas		1.193
Rpim	0.030	0.330
Number of reflections	15019
<I/σ(I)>	19.8	2.3
Completeness [%]	99.0	97.41
Redundancy	12.9	12.66
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate