5OTT
Extracellular domain of GLP-1 receptor in complex with exendin-4 variant Gly2Hcs/Thr5Hcs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS3 R 1M |
| Wavelength(s) | 1.54187 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.690, 62.920, 118.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.382 - 1.920 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.382 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.103 | 1.145 |
| Rmeas | 1.193 | |
| Rpim | 0.030 | 0.330 |
| Number of reflections | 15019 | |
| <I/σ(I)> | 19.8 | 2.3 |
| Completeness [%] | 99.0 | 97.41 |
| Redundancy | 12.9 | 12.66 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Imidazole pH 6.5, 1.0 M Sodium acetate trihydrate |
