5OSB
GLIC-GABAAR alpha1 chimera crystallized in complex with THDOC at pH4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.218, 133.507, 162.263 |
Unit cell angles | 90.00, 103.41, 90.00 |
Refinement procedure
Resolution | 29.980 - 3.800 |
R-factor | 0.244 |
Rwork | 0.241 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5osa |
RMSD bond length | 0.001 |
RMSD bond angle | 0.432 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.030 | 3.970 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.185 | |
Number of reflections | 37899 | |
<I/σ(I)> | 4.6 | |
Completeness [%] | 99.9 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 12-16% PEG4000, 100mM NaCl, 100 mM Li2SO4, 100mM sodium acetate pH4-4.5 |