5OO6

Complex of human nuclear cap-binding complex with ARS2 C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	70.520, 112.990, 270.980
Unit cell angles	90.00, 90.30, 90.02

Refinement procedure

Resolution	270.980 - 2.800
R-factor	0.23236
Rwork	0.231
R-free	0.26798
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2t
RMSD bond length	0.008
RMSD bond angle	1.194
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	280.000	2.910
High resolution limit [Å]	2.800	2.800
Number of reflections	200837	21706
<I/σ(I)>	6.63	1.2
Completeness [%]	97.5	96.3
Redundancy	2.08	2
CC(1/2)	0.995	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	CBC was mixed with an access of ARS2(827-871) in the presence of 1 mM m7GTP and subjected to gel filtration (120 mM NaCl, 5 mM beta-mercaptoethanol, 20 mM HEPES pH 7.8). The complex was concentrated to 8 mg/ml. Crystals were obtained in mother liquor containing 0.1 M sodium acetate pH 5, 8% (v/v) MPD and 0.1 M guanidine hydrochloride at 20 C.