5ONC
Catabolism of the Cholesterol Side Chain in Mycobacterium tuberculosis is Controlled by a Redox-Sensitive Thiol Switch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 120.289, 120.289, 206.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.170 - 2.190 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.22568 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ubw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.619 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | 2.260 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 46018 | 3897 |
| <I/σ(I)> | 15.2 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 9.7 |
| CC(1/2) | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M citrate, pH 5.5, 0.7 M (NH4)2HPO4, 0.3 M NaCl |
