5OMV
Ternary complex of 9N DNA polymerase in the replicative state with two metal ions in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.999973488495 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.688, 94.964, 158.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.482 - 2.003 |
| R-factor | 0.1916 |
| Rwork | 0.189 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k8x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12rc0_2787: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.482 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.142 | 3.603 |
| Number of reflections | 130652 | 21057 |
| <I/σ(I)> | 7.88 | 0.37 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M sodium acetate trihydrate pH 4.5, 6% isopropanol, 36% PEG 550 MME |
