5OMF
Closed, ternary structure of KOD DNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999766 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.909, 147.559, 71.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.266 - 2.092 |
R-factor | 0.1973 |
Rwork | 0.195 |
R-free | 0.23350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k8z |
RMSD bond length | 0.002 |
RMSD bond angle | 0.527 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12rc1_2815: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.190 | 2.220 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmeas | 0.185 | 1.720 |
Number of reflections | 127923 | |
<I/σ(I)> | 6.37 | 0.81 |
Completeness [%] | 98.8 | 95.8 |
Redundancy | 3.56 | 3.41 |
CC(1/2) | 0.993 | 0.285 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M DL-glutamatic acid, 0.2 M DL-alanine, 0.2 M glycine, 0.2 M DL-lysine monohydrochloride, 0.2 M DL-serine, 0.1 M Hepes/ 0.1 M Mops, 20 % glycerol, 10 % PEG 4000 |