5OKZ

Crystal Strucrure of the Mpp6 Exosome complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.25
Spacegroup name	P 1 21 1
Unit cell lengths	161.779, 237.430, 201.895
Unit cell angles	90.00, 110.37, 90.00

Refinement procedure

Resolution	80.760 - 3.200
R-factor	0.224490359891
Rwork	0.223
R-free	0.26152
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jea
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	151.660	3.250
High resolution limit [Å]	3.200	3.200
Rmerge	0.214	1.881
Rpim	0.114	1.298
Number of reflections	229497
<I/σ(I)>	5.7	0.6
Completeness [%]	97.7	79.9
Redundancy	4.3	2.8
CC(1/2)	0.985	0.243

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M Tris/Mops pH 7.5 30 mM MgCl2, 30 mM CaCl2 30% PEG 8000/Ethylene glycol