5OKT
Crystal structure of human Casein Kinase I delta in complex with IWP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.110, 82.730, 89.640 |
| Unit cell angles | 89.73, 74.14, 86.57 |
Refinement procedure
| Resolution | 47.155 - 2.130 |
| R-factor | 0.1939 |
| Rwork | 0.193 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mqv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.715 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.155 |
| High resolution limit [Å] | 2.130 |
| Number of reflections | 74219 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 98.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 5.0 and 5 % (w/v) PEG 2000 MME |
