5OKN

Crystal structure of human SHIP2 Phosphatase-C2 D607A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.872600
Spacegroup name	P 21 21 21
Unit cell lengths	137.065, 177.143, 177.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.170 - 2.650
R-factor	0.20796
Rwork	0.206
R-free	0.24463
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.267
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.170	2.700
High resolution limit [Å]	2.650	2.650
Rpim	0.055	0.467
Number of reflections	119204
<I/σ(I)>	13.4	2.2
Completeness [%]	100.0	100
Redundancy	9	8.9
CC(1/2)	0.996	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Bis-Tris propane, pH 7.0, 0.4 M KSCN, 20% (w/v) PEG3350