5OJ6
Crystal structure of the chicken MDGA1 ectodomain in complex with the human neuroligin 1 (NL1(-A-B)) cholinesterase domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.420, 184.140, 96.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.362 - 3.300 |
| R-factor | 0.2294 |
| Rwork | 0.227 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oj2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.939 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2044:)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.070 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmeas | 1.063 | |
| Number of reflections | 29576 | 2080 |
| <I/σ(I)> | 15.6 | 1.6 |
| Completeness [%] | 98.7 | 95.4 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.999 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Na.HEPES pH 7.0, 7.5% w/v PEG8000 |
