5OJ6

Crystal structure of the chicken MDGA1 ectodomain in complex with the human neuroligin 1 (NL1(-A-B)) cholinesterase domain.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Neuroligin-1	polymer	575	64397.3	1	UniProt (Q8N2Q7) Pfam (PF00135)	Homo sapiens (Human)
2	B (B)	MAM domain-containing glycosylphosphatidylinositol anchor protein 1	polymer	910	102143.9	1	UniProt (Q0WYX8) Pfam (PF13927) Pfam (PF00629)	Gallus gallus (Chicken)
3	C (C)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	D, E, F, G, H... (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)

Sequence modifications

A: 46 - 635 (UniProt: Q8N2Q7)

PDB	External Database	Details
Thr 44	-	expression tag
Gly 45	-	expression tag
-	Gly 278	deletion
-	Asn 279	deletion
-	Arg 280	deletion
-	Trp 281	deletion
-	Ser 282	deletion
-	Asn 283	deletion
-	Ser 284	deletion
-	Thr 285	deletion
-	Lys 286	deletion
Arg 636	-	expression tag
Thr 637	-	expression tag
Lys 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag

B: 19 - 919 (UniProt: Q0WYX8)

PDB	External Database	Details
Lys 120	Arg 120	engineered mutation
Gly 920	-	expression tag
Thr 921	-	expression tag
Lys 922	-	expression tag
His 923	-	expression tag
His 924	-	expression tag
His 925	-	expression tag
His 926	-	expression tag
His 927	-	expression tag
His 928	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	166541.1
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	8
	Total formula weight	1769.7
All*	Total formula weight	168897.3

*Water molecules are not included.