5OEN
Crystal Structure of STAT2 in complex with IRF9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.602, 124.037, 51.029 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 32.120 - 2.919 |
| R-factor | 0.26 |
| Rwork | 0.255 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bf5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.463 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.389 | 2.930 |
| High resolution limit [Å] | 2.919 | 2.919 |
| Rmerge | 0.194 | 0.898 |
| Number of reflections | 7970 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 96.0 | 98.7 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.2M Potassium formate and 20% PEG3350 |
