5ODT
Aurora-A in complex with TACC3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 137.040, 137.040, 137.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.520 - 2.021 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ceg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.047 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.520 | 2.070 |
| High resolution limit [Å] | 2.021 | 2.020 |
| Rmerge | 0.087 | 0.193 |
| Number of reflections | 28159 | |
| <I/σ(I)> | 25.9 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 20.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.1 M Bis-Tris pH 5.5, 2 M Ammonium sulphate |
