5OC4
Crystal structure of human tRNA-dihydrouridine(20) synthase dsRBD R361A-R362A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | H 3 |
| Unit cell lengths | 83.940, 83.940, 56.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.970 - 1.710 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wft |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.520 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.217 | |
| Number of reflections | 16219 | 625 |
| <I/σ(I)> | 7 | 1.02 |
| Completeness [%] | 91.0 | 39.01 |
| Redundancy | 5.6 | 5.9 |
| CC(1/2) | 0.992 | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 22% PEG 8K 100 mM sodium cacodylate |
