5O9M

Crystal structure of human Histamine-Releasing Factor (HRF/TCTP)containing a disulphide-linked dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	47.527, 77.592, 99.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.855 - 1.400
R-factor	0.1803
Rwork	0.179
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yz1
RMSD bond length	0.015
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	1.430
High resolution limit [Å]	1.400	1.400
Rmerge	0.066	1.112
Rpim	0.025	0.428
Number of reflections	72800	3759
<I/σ(I)>	12.7
Completeness [%]	99.7	99.6
Redundancy	7.4	7.6
CC(1/2)	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1M MES pH6.0 and 20% (w/v) PEG MME 2000