5O90
Crystal structure of a P38alpha T185G mutant in complex with TAB1 peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.920, 73.580, 58.970 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 58.959 - 2.490 |
| R-factor | 0.2502 |
| Rwork | 0.249 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4loo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.179 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.960 | 2.320 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.054 | 0.852 |
| Number of reflections | 18410 | 1340 |
| <I/σ(I)> | 22 | 1.1 |
| Completeness [%] | 99.5 | 93.6 |
| Redundancy | 9.4 | 3.7 |
| CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20% PEG3350 0.2M Na/K Tartrate 0.1M pH7.0 Bis-Tris Propane |
