5O6T

BIRC4 RING in complex with dimeric ubiquitin variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	110
Detector technology	PIXEL
Collection date	2016-04-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	35.715, 69.972, 109.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.180 - 1.570
R-factor	0.158
Rwork	0.156
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eb5 1ubq
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.970	1.577
High resolution limit [Å]	1.570	1.570
Rmerge	0.074	0.890
Rpim	0.042	0.513
Number of reflections	39227	2560
<I/σ(I)>	14.9	2
Completeness [%]	100.0	96.3
Redundancy	6.4	6.5
CC(1/2)		0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	291	0.1 M NaHEPES pH 7.5, 1.4 M tri-Na citrate