5NX2

Crystal structure of thermostabilised full-length GLP-1R in complex with a truncated peptide agonist at 3.7 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 31 2 1
Unit cell lengths	94.435, 94.435, 163.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.677 - 3.700
R-factor	0.2872
Rwork	0.285
R-free	0.33410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iol 5ee7
RMSD bond length	0.004
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1-2575-000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.600	4.050
High resolution limit [Å]	3.700	3.700
Rmerge	0.134	2.024
Rpim	0.073	1.078
Number of reflections	9266	2205
<I/σ(I)>	6.6	1.2
Completeness [%]	98.5	99.7
Redundancy	6.6	6.9
CC(1/2)	0.998	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.1M Tris-HCl pH 8.0-9.0, 32-44% (v/v) PEG 200