5NVK

Crystal structure of the human 4EHP-GIGYF1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.999810
Spacegroup name	P 42
Unit cell lengths	135.320, 135.320, 60.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.843 - 2.900
R-factor	0.2067
Rwork	0.204
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nvl
RMSD bond length	0.003
RMSD bond angle	0.494
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.843	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	24699
<I/σ(I)>	10.9	1.95
Completeness [%]	99.6	99.9
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG 3350 0.2 M KCl