5NTD
Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Bestatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.260, 143.450, 268.030 |
| Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
| Resolution | 80.330 - 2.300 |
| R-factor | 0.18995 |
| Rwork | 0.189 |
| R-free | 0.21375 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nsk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.475 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.330 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.091 | 0.523 |
| Number of reflections | 290030 | |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.0, 40 % (v/v) MPD, 5 mM MnCl2, 5 mM bestatin |
