5NSC

Fc DEKK heterodimer variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	60.059, 65.442, 78.710
Unit cell angles	90.00, 103.24, 90.00

Refinement procedure

Resolution	76.619 - 2.300
R-factor	0.2273
Rwork	0.225
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dk0
RMSD bond length	0.003
RMSD bond angle	0.725
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.620	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.154	0.531
Rpim	0.106	0.367
Number of reflections	26379	2511
<I/σ(I)>	3.6	1.7
Completeness [%]	98.7	95.4
Redundancy	2.8	2.9
CC(1/2)	0.972	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M sodium fluoride, 20% w/v PEG3350