5NHR

CRYSTAL STRUCTURE OF THE Activin receptor type-2B LIGAND BINDING DOMAIN IN COMPLEX WITH BIMAGRUMAB FV, CUBIC CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 2 3
Unit cell lengths	187.826, 187.826, 187.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.350
Rwork	0.216
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h64
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.1)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.470
High resolution limit [Å]	3.350	7.220	3.350
Rmerge	0.093	0.032	0.482
Total number of observations	96966
Number of reflections	28995
<I/σ(I)>	8.2
Completeness [%]	90.6	84	93.9
Redundancy	3.3	3.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	1.4M AMMONIUM SULFATE, 0.1M SODIUM CITRATE