5NHR
CRYSTAL STRUCTURE OF THE Activin receptor type-2B LIGAND BINDING DOMAIN IN COMPLEX WITH BIMAGRUMAB FV, CUBIC CRYSTAL FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 187.826, 187.826, 187.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.350 |
| Rwork | 0.216 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h64 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.1) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 7.220 | 3.350 |
| Rmerge | 0.093 | 0.032 | 0.482 |
| Total number of observations | 96966 | ||
| Number of reflections | 28995 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 90.6 | 84 | 93.9 |
| Redundancy | 3.3 | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 1.4M AMMONIUM SULFATE, 0.1M SODIUM CITRATE |
