5NH3
CRYSTAL STRUCTURE OF THE Activin receptor type-2A LIGAND BINDING DOMAIN IN COMPLEX WITH BIMAGRUMAB FV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99992 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.330, 108.207, 100.685 |
| Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
| Resolution | 47.550 - 2.350 |
| R-factor | 0.1679 |
| Rwork | 0.166 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ngv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.089 | 0.599 |
| Number of reflections | 38338 | |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 97.9 | 97.3 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium citrate tribasic dihydrate pH3.5 25% PEG 3350 |
