5NFU
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with LHSpTA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.197, 59.808, 58.256 |
| Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
| Resolution | 41.199 - 1.809 |
| R-factor | 0.1699 |
| Rwork | 0.168 |
| R-free | 0.20200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.808 | 1.815 |
| High resolution limit [Å] | 1.809 | 1.809 |
| Rmerge | 0.448 | |
| Rpim | 0.044 | 0.314 |
| Number of reflections | 21491 | 531 |
| <I/σ(I)> | 3.7 | 2.2 |
| Completeness [%] | 99.3 | 90.8 |
| Redundancy | 3.7 | 2.7 |
| CC(1/2) | 0.998 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M HEPES 7 30% Jeffamine ED-2001 |
