5NFN
JMJD7 IN COMPLEX WITH MN AND 2OG IN THE H32 FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 207.220, 207.220, 211.788 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.459 - 2.982 |
| R-factor | 0.24 |
| Rwork | 0.249 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 (706E) |
| Data scaling software | HKL-2000 (706E) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.459 | 3.070 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.169 | 1.296 |
| Number of reflections | 35671 | |
| <I/σ(I)> | 10.3 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 25 mg/mL His6-JMJD7, 2 mM MnCl2, 4 mM 2OG, 1.5 M Sodium Nitrate, 0.1 M Sodium Acetate pH 4.6 |
