5NBM

Crystal structure of the Arp4-N-actin(ATP-state) heterodimer bound by a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	190.581, 190.581, 220.616
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.735 - 3.400
R-factor	0.1539
Rwork	0.152
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.659
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.230	49.230	3.490
High resolution limit [Å]	3.400	15.210	3.400
Rmerge	0.165	0.036	1.113
Rmeas	0.180	0.040	1.206
Rpim	0.070	0.016	0.463
Total number of observations	405414
Number of reflections	62339
<I/σ(I)>	11.3
Completeness [%]	99.9	96.5	100
Redundancy	6.5	5.9	6.8
CC(1/2)	0.996	0.999	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1 mM ATP, 0.2 mM CaCl2, 1.3-1.5 M sodium malonate pH 6.0, Subtilisin (1/6000; w(subtilisin)/w(protein))